天然产物生物合成与智能设计团队

发布人：学院发布时间：2025-02-21 点击：

一、团队名称

天然产物生物合成与智能设计团队（PI：刘晓楠）

二、团队组成

团队目前有教师4人（刘晓楠、王永泽、赵锦芳、高娃），其中教授/博导1人、副教授2人、讲师1人。

三、团队介绍

团队主要研究方向是天然产物合成生物学。

研究内容：针对珍稀药用天然产物及合成关键后修饰酶，从“生物催化元件的功能筛选与智能设计”和“人工合成途径的系统创建与过程优化”两方面开展系统研究：一方面基于多组学分析方法，自主开发自然元件挖掘平台，系统构建后修饰酶基础数据库；另一方面基于理性设计和深度学习理论，开发人工元件设计平台，打破进化局限，为珍稀药用天然产物的生物合成提供高效催化元件。

团队基于在合成生物学、有机化学、酶工程和生物信息学、计算生物学、人工智能等领域的良好研究基础，已经挖掘并解析了多种后修饰酶（P450酶、甲基转移酶、糖基转移酶、异戊烯基转移酶）的分子基础，实现了心脑血管疾病特效药物灯盏乙素、镇咳药物虎杖苷、抗癌药物紫杉醇中间体等20余种天然产物的途径解析和生物合成。在Molecular Plant、Nature Communications、Research、Plant Communications、Science China Life Sciences、PloS Biology等期刊发表学术论文24篇。申请发明专利10项，已授权7项。

四、科研成果

（1） 代表性科研项目

1.国家自然科学基金面上项目：紫杉二烯-5α-羟化酶催化混杂性机制解析与理性设计，32371499，项目日期：2024.01-2027.12

2.国家自然科学基金青年基金项目：黄酮8-O-甲基转移酶功能解析与催化机理研究，31901026，项目日期：2020.01-2022.12

3.中国博士后科学基金项目：紫杉醇生物合成机制的研究，213371，项目日期：2019.11-2021.11

4.国家重点研发计划：甾体激素高效合成人工细胞的创建与优化，2019YFA0905303，项目日期：2020.01-2024.12

5.国家重点研发计划：紫杉醇生物合成未知基因鉴定及途径解析，2020YFA0908000，项目日期：2020.11-2025.10

6.国家自然科学基金青年基金项目：氯虫苯甲酰胺高效降解菌株GW13降解基因的克隆及功能研究，31501677，项目日期：2016.01-2018.12

7.湖北省教育厅重点项目，球孢白僵菌高毒力菌株筛选及制剂开发与应用研究，D20201403，2020.1-2021.12

8.农业部华中作物有害生物综合治理重点实验室开放基金，2019ZTSJJ3，杀虫真菌僵蚕白僵菌高效菌株筛选及应用研究，2019.11-2021.10

9.企业合作项目：高效利用蔗糖生产L-乳酸的大肠杆菌及应用，2024307，2024.07-2024.12

10.企业合作项目：基于代谢通量分析改造丁二酸工程菌的研究，2021433，2021.08-2022.08

（2） 代表性论文：(2017-，“*”为共同第一作者，"#"为通讯作者)

1. Xiaonan Liu*, Jian Cheng*, Guanghui Zhang*, Wentao Ding, Lijin Duan, Jing Yang, Ling Kui, Xiaozhi Cheng, Jiangxing Ruan, Wei Fan, Junwei Chen, Guangqiang Long, Yan Zhao, Jing Cai, Wen Wang, Yanhe Ma, Yang Dong, Shengchao Yang# and Huifeng Jiang#. Engineering yeast for the production of breviscapine by genomic analysis and synthetic biology approaches.Nature Communications 2018, 9 (1): 448. (IF: 16.6, Q1)

2. Jian Cheng*, Xiao Wang*, Xiaonan Liu*, Xiaoxi Zhu*, Zihe Li, Huanyu Chu, Qian Wang, Qianqian Lou, Bijun Cai, Yiqun Yang, Xiaoyun Lu, Kai Peng, Dingyu Liu, Yuwan Liu, Lina Lu, Huan Liu, Ting Yang, Qijin Ge, Chengcheng Shi, Guichun Liu, Zhiwei Dong, Xun Xu#, Wen Wang#, Huifeng Jiang# and Yanhe Ma. Chromosome-level genome of Himalayan yew provides insights into the origin and evolution of the paclitaxel biosynthetic pathway.Molecular Plant 2021, 14(7): 1199-1209. (IF: 27.5, Q1)

3. Yuqian Liu*, Qian Wang*,Xiaonan Liu*, Jian Cheng*#, Lei Zhang, Huanyu Chu, Ruoyu Wang, Haoran Li, Hong Chang, Nida Ahmed, Zhonghua Wang, Xiaoping Liao# and Huifeng Jiang#. pUGTdb: A comprehensive database of plant UDP-dependent glycosyltransferases.Molecular Plant 2023, S1674-2052(23)00003-5. (IF: 27.5, Q1)

4. Qian Wang*,Xiaonan Liu*, Hejian Zhang*, Huanyu Chu*, Chao Shi*, Lei Zhang, Jie Bai, Pi Liu, Jing Li, Xiaoxi Zhu, Yuwan Liu, Zhangxin Chen, Rong Huang, Hong Chang, Tian Liu, Zhenzhan Chang#, Jian Cheng#, Huifeng Jiang#. Cytochrome P450 Enzyme Design by Constraining Catalytic Pocket in Diffusion Model.Research 2024, 8:7:0413. (IF: 11.0, Q1)

5. Bihuan Chen*,Xiaonan Liu*, Yina Wang*, Jie Bai*, Xiangyu Liu, Guisheng Xiang, Wei Liu, Xiaoxi Zhu, Jian Cheng, Lina Lu, Guanghui Zhang, Shuhui Zi#, Shengchao Yang# and Huifeng Jiang#. Production of the antidepressant orcinol glucoside in Yarrowia lipolytica with yields over 6,400-fold higher than plant extraction.Plos biology2023, 21(6): e3002131. (IF: 9.8, Q1)

6.Xiaonan Liu*#, Xiaoxi Zhu, Jian Cheng, Huifeng Jiang#. A new era for paclitaxel biosynthesis is coming.Molecular Plant 2024, 17(3):370-371. (IF: 27.5, Q1)

7.Xiaonan Liu*, Jian Cheng*, Xiaoxi Zhu, Guanghui Zhang, Shengchao Yang, Xiaoxian Guo#, Huifeng Jiang# and Yanhe Ma. De Novo Biosynthesis of Multiple Pinocembrin Derivatives in Saccharomyces cerevisiae.ACS Synthetic Biology 2020, 9 (11): 3042-3051. (IF: 4.7, Q2)

8. Jing Li*,Xiaonan Liu*#, Xiaoxi Zhu, Jiayu Liu, Lei Zhang, Nida Ahmed, Jian Qi, Bihuan Chen, Daliang Tang, Jinsheng Yu, Zhijin Fan#, Huifeng Jiang#. Biochemical synthesis of taxanes from mevalonate.Synthetic and Systems Biotechnology 2024, 9 (2024): 694–700. (IF: 4.8, Q2)

9. Chunmei Jiang*,Xiaonan Liu*, Xianqing Chen, Yi Cai, Yibin Zhuang, Tao Liu, Xiaoxi Zhu, Hui Wang, Yuwan Liu, Huifeng Jiang and Wen Wang#. Raising the production of phloretin by alleviation of by-product of chalcone synthase in the engineered yeast.Science China Life sciences 2020, 63(11): 1734-1743. (IF: 9.1, Q1)

10. Xiaoxi Zhu*,Xiaonan Liu*, Tian Liu, Yina Wang, Nida Ahmed, Zhichao Li# and Huifeng Jiang#. Synthetic biology of plant natural products: From pathway elucidation to engineered biosynthesis in plant cells.Plant Communications 2021,2(5): 100229. (IF: 10.5, Q1)

11.Xiaonan Liu*, Xiaoxi Zhu*, Hui Wang, Tian Liu, Jian Cheng and Huifeng Jiang#. Discovery and modification of cytochrome P450 for plant natural products biosynthesis.Synthetic and systems biotechnology 2020, 5 (3): 187-199. (IF: 4.8, Q2)

12. Jian Cheng*, Jing Chen*,Xiaonan Liu*, Xiangchen Li, Weixiong Zhang, Zhubo Dai, Lina Lu, Xiang Zhou, Jing Cai#, Xueli Zhang#, Huifeng Jiang# and Yanhe Ma. The origin and evolution of the diosgenin biosynthetic pathway in yam.Plant Communications 2020,2(1): 100079. (IF: 10.5, Q1)

13. Tian Liu*, Yuqian Liu*, Lan Li*,Xiaonan Liu*, Zhaokuan Guo, Jian Cheng,Xiaoxi Zhu, Lina Lu, Junlin Zhang, Guanwei Fan, Nengzhong Xie#, Jian Lu# and Huifeng Jiang#. De Novo Biosynthesis of Polydatin in Saccharomyces cerevisiae.Journal of agricultural and food chemistry 2021, 69(21): 5917-5925. (IF: 6.1, Q1)

14. Yina Wang*#,Xiaonan Liu*#, Bihuan Chen, Wei Liu, Zhaokuan Guo, Xiangyu Liu, Xiaoxi Zhu, Jiayu Liu, Jin Zhang, Jing Li, Lei Zhang, Yadi Gao, Guanghui Zhang, Yan Wang, M. Iqbal Choudhary, Shengchao Yang# and Huifeng Jiang#. Metabolic engineering of Yarrowia lipolytica for scutellarin production.Synthetic and Systems Biotechnology 2022, 7(3): 958-964. (IF: 4.8, Q2)

15. Xiangchen Li*, Jian Cheng*,Xiaonan Liu*, Xiaoxian Guo, Yuqian Liu, Wenjing Fan, Lina Lu, Yanhe Ma, Tao Liu, Shiheng Tao# and Huifeng Jiang#. Origin and Evolution of Fusidane-Type Antibiotics Biosynthetic Pathway through Multiple Horizontal Gene Transfers.Genome Biology and Evolution 2020, 12 (10): 1830-1840. (IF: 3.3, Q2)

16.Xiaonan Liu*, Wentao Ding* and Huifeng Jiang#. Engineering microbial cell factories for the production of plant natural products: from design principles to industrial-scale production.Microbial Cell Factories 2017, 16 (1): 125. (IF: 6.4, Q2)

17. Lijin Duan*, Wentao Ding*,Xiaonan Liu, Xiaozhi Cheng, Jing Cai, Erbing Hua#, Huifeng Jiang#. Biosynthesis and engineering of kaempferol in Saccharomyces cerevisiae.Microbial Cell Factories 2017, 16 (1):165. (IF: 6.4, Q2)

18. Xitong Song*, Qian Wang*, Xiaoxi Zhu*, Wenhan Fang*,Xiaonan Liu, Chao Shi, Zhenzhan Chang#, Huifeng Jiang#, and Binju Wang#. Unraveling the Catalytic Mechanism of Taxadiene-5α-hydroxylase from Crystallography and Computational Analyses.ACS Catalysis 2024, 14, 3912−3925. (IF: 12.9, Q2)

19.刘晓楠*#，李静，祝晓熙，徐子硕，程健，江会锋#．紫杉醇生物合成机制研究进展．合成生物学2024，5:1-21.

20.毕慧萍*，刘晓楠*，李清艳，程健，庄以彬，王冬，戴住波，江会锋，刘涛#，张学礼#.植物天然产物微生物重组合成研究进展.生物工程学报 2022, 38(11): 4263-4282.

21.Jinfang Zhao*, Wenlu Song, Jun Cheng#, Min Liu, Chuanxi Zhang, Kefa Cen. Improvement of fermentative hydrogen production using genetically modifiedEnterobacter aerogenes. International Journal of Hydrogen Energy. 2017, 42: 3676-3681. (IF:8.1, Q2)

22. 黄满*，李焕梅，胡靖，陈智勇，危俊杰，龙同，宋满，赵锦芳^#．两株芽孢杆菌脂肽粗提物对马铃薯致病疫霉抑菌效果的研究[J]．食品与发酵工业，2025，51 ( 3) : 97-103．

23. 李敏*，黄满，邱炜玥，李焕梅，贾子民，龙同，赵锦芳^#.响应面法优化卡氏芽孢杆菌Bacillus cabrialesii ST-1发酵培养基.中国酿造. 2023, 42(2): 193-198.

24.Wa Gao*, Dongyang Li, Hong You^#. Functional Characterization and Genomic Analysis of the Chlorantraniliprole-Degrading Strain Pseudomonas Sp. GW13, Bioengineering, 2019, 6: 106. (IF:4.6, Q3)

五、研究生毕业去向

1) 攻读博士学位：