一、团队名称
结构生物学与分子医学团队
二、团队组成
本团队科研教师4人（潘军华、陈明月、王永胜等），其中教授/博导1人、副教授1人、讲师1人、科研助理1人。
三、团队PI及团队介绍
潘军华，结构生物学、病毒学、抗体免疫、生物化学、生物物理学专家。1999年于武汉工业学院获学士学位，2002年于江南大学获硕士学位，同年获全额奖学金赴美国Rice University留学，2008年获哲学博士学位获颁George J.Schroepfer,Jr.杰出研究奖。2008-2009年为Rice University Research Associate,2009起师从哈佛医学院Stephen C.Harrison,为HHMI博士后研究员，2011-2022为波士顿儿童医院Research Associate。以第一或通讯作者在Nature，Science，Cell，PANS，Structure，J Struct Biol，JMB，J Virol，Virology等发表论文10余篇，拥有欧洲专利1项。担任Nature Communications,Communications Biology,Bioconjuate Chemistry等杂志审稿人。曾组织学术会议促进学术界与工业界交流与合作。
团队介绍
本团队主要运用冷冻电镜及X射线晶体衍射等方法解析生物大分子的高分辨率结构，并结合生物化学、生物物理学、细胞生物学、生物信息学、及大数据分析等方法，揭示重大人类病原入侵宿主细胞、基因组复制和转录、病原与宿主尤其免疫系统相互作用等的分子机制，为新药开发、新工具设计、临床诊断等提供基础。团队目前有PI一名，教师两名，科研助理一名，博士及硕-博连读生两名，硕士生十三名，及基地班学生两名。
研究方向：1）病毒基因的组织、复制/逆转录、转录及其调控；2）病毒的入侵及膜融合；3）蛋白质及病毒设计与改造；4）抗体免疫与疫苗设计。
四、科研成果
1. 代表性科研项目：
(1) 省自然基金重点项目，ZXKY20240180，猴痘病毒疫苗设计及新型药物开发
(2) 国家重点研发计划项目子课题，2022YFC2604000，复杂现场模拟仿真技术研究
(3) 国家自然科学基金青年项目，82303528，HERVs来源的新的lncRNA——HILAL在肝癌发展中的作用及分子机制研究
(4) 武汉市自然科学基金探索计划，2024040801020320，PD-1免疫药物在肝癌临床治疗上产生疗效差异的内在机理研究
(5) 湖北省自然科学基金青年项目, 2021CFB274,人内源性逆转录病毒在乳腺癌中的激活表达及其功能机制研究
(6) 国家细胞调控与分子药物学科创新引智基地（“111计划”），XBTK-2024009，10-HDA mitigates TMT-induced disruption of meiotic maturation in mouse oocytes
(7) 发酵工程教育部重点实验室开放基金，202409FE17，王浆酸缓解全氟烷基醚磺酸钾（F-53B）暴露导致卵母细胞发育异常的机制研究
2. 代表性论文：(“*”为通讯作者)
(1) Shankar S#,Pan J#, Yang P#, Bian Y#, Oroszlán G, Yu Z, Mukherjee P, Filman DJ, Hogle JM, Shekhar M, Coen DM, Abraham J*. Viral DNA polymerase structures reveal mechanisms of antiviral drug resistance.Cell.2024,187(20):5572-5586.e15.
(2) Yang P, Li W, Fan X, Pan J, Mann CJ, Varnum H, Clark LE, Clark SA, Coscia A, Basu H, Smith KN, Brusic V, Abraham J. Structural basis for VLDLR recognition by eastern equine encephalitis virus.Nat Commun. 2024,15(1):6548.
(3) Nabel KG#, Clark SA#, Shankar S#, Pan J#, Clark LE, Yang P, Coscia A, McKay LGA, Varnum HH, Brusic V, Tolan NV, Zhou G, Desjardins M, Turbett SE, Kanjilal S, Sherman AC, Dighe A, LaRocque RC, Ryan ET, Tylek C, Cohen-Solal JF, Darcy AT, Tavella D, Clabbers A, Fan Y, Griffiths A, Correia IR, Seagal J, Baden LR, Charles RC, Abraham J*. Structural basis for continued antibody evasion by the SARS-CoV-2 receptor binding domain.Science. 2022,375(6578):eabl6251.
(4) Clark SA#, Clark LE#,Pan J#, Coscia A, McKay LGA, Shankar S, Johnson RI, Brusic V, Choudhary MC, Regan J, Li JZ, Griffiths A, Abraham J*. SARS-CoV-2 evolution in an immunocompromised host reveals shared neutralization escape mechanisms.Cell. 2021,184(10):2605-2617.e18.
(5) Pan J, Peng H, Chen B, Harrison SC*. Cryo-EM Structure of Full-length HIV-1 Env Bound With the Fab of Antibody PG16.J Mol Biol. 2020,432(4):1158-1168.
(6) Pan J#*, Qian X#, Lattmann S, El Sahili A, Yeo TH, Jia H, Cressey T, Ludeke B, Noton S, Kalocsay M, Fearns R*, Lescar J*. Structure of the human metapneumovirus polymerase phosphoprotein complex.Nature. 2020,577(7789):275-279.
(7) Liu Y,Pan J, Jenni S, Raymond DD, Caradonna T, Do KT, Schmidt AG, Harrison SC*, Grigorieff N*. CryoEM Structure of an Influenza Virus Receptor-Binding Site Antibody-Antigen Interface.J Mol Biol. 2017,429(12):1829-1839.
(8) Liu Y#*,Pan J#, Cai Y, Grigorieff N, Harrison SC, Chen B. Conformational States of a Soluble, Uncleaved HIV-1 Envelope Trimer.J Virol. 2017,91(10):e00175-17.
(9) Campbell MG#, Cheng A#, Brilot AF, Moeller A, Lyumkis D, Veesler D,Pan J, Harrison SC, Potter CS, Carragher B, Grigorieff N*. Movies of ice-embedded particles enhance resolution in electron cryo-microscopy.Structure. 2012,20(11):1823-8.
(10) Brilot AF, Chen JZ, Cheng A,Pan J, Harrison SC, Potter CS, Carragher B, Henderson R, Grigorieff N*. Beam-induced motion of vitrified specimen on holey carbon film.J Struct Biol. 2012,177(3):630-7.
(11) Pan J, Dong L, Lin L, Ochoa WF, Sinkovits RS, Havens WM, Nibert ML, Baker TS, Ghabrial SA, Tao YJ. Atomic structure reveals the unique capsid organization of a dsRNA virus. Proc Natl Acad Sci U S A. 2009,106(11):4225-30.
(12) Chen M#, Jia L#, Zheng X, Han M, Li L*, Zhang L*. Ancient Human Endogenous Retroviruses Contribute to Genetic Evolution and Regulate Cancer Cell Type-Specific Gene Expression.Cancer Res. 2022,82(19):3457-3473.
(13) Zhang L#, Geng X#, Wang F#, Tang J, Ichida Y, Sharma A, Jin S,Chen M, Tang M, Pozo FM, Wang W, Wang J, Wozniak M, Guo X, Miyagi M, Jin F, Xu Y, Yao X, Zhang Y*. 53BP1 regulates heterochromatin through liquid phase separation.Nat Commun. 2022,13(1):360.
(14) Chen M#, Guo X, Zhang L*. Unexpected Discovery and Expression of Amphibian Class II Endogenous Retroviruses. J Virol. 2021,95(3):e01806-20.
(15) Wang YS#, Yang SJ#, Wan ZX, Shen A, Ahmad MJ, Chen MY, Huo LJ, Pan JH*. Chlorothalonil exposure compromised mouse oocyte in vitro maturation through inducing oxidative stress and activating MAPK pathway.Ecotoxicol Environ Saf. 2024,273:116100.
(16) Wang YS, Chen C, Ahmad MJ, Chen F, Ding ZM, Yang SJ, Chen YW, Duan ZQ, Liu M, Liang AX, He CJ, Hua GH*, Huo LJ*. WDR62 regulates mouse oocyte meiotic maturation related to p-JNK and H3K9 trimethylation.Int J Biochem Cell Biol. 2022,144:106169.
(17) Wang YS, Jiao XF, Chen F, Wu D, Ding ZM, Miao YL, Huo LJ. WDR62 is a novel participator in spindle migration and asymmetric cytokinesis during mouse oocyte meiotic maturation.Exp Cell Res. 2020,387(1):111773.
(18) Wang YS, Yang SJ, Ahmad MJ, Ding ZM, Duan ZQ, Chen YW, Liu M, Liang AX, Hua GH*, Huo LJ*. Zinc pyrithione exposure compromises oocyte maturation through involving in spindle assembly and zinc accumulation.Ecotoxicol Environ Saf. 2022,234:113393.
(19) Wang YS, Zhang LD, Zhou X, Yang SJ, Ahmad MJ, Ding ZM, Duan ZQ, Chen YW, Liu M, Zhang SX, Liang AX, Hua GH*, Huo LJ*. Bromoacetic acid impairs mouse oocyte in vitro maturation through affecting cytoskeleton architecture and epigenetic modification.Chem Biol Interact. 2022,368:110192.
五、联系方式
联系人：王永胜
邮箱：wangyongsheng@hbut.edu.cn
